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xmg1 2  (Bio X Cell)


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    Structured Review

    Bio X Cell xmg1 2
    Xmg1 2, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 250 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xmg1+2/InVivoPlus+anti-mouse+IFN%CE%B3/10__1158_slash_2326___6066__cir___25___0651-66-62-63
    Average 96 stars, based on 250 article reviews
    xmg1 2 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Mouse Assay:

    Article Title: IL-1R2 Deficiency Unleashes Neutrophil-Mediated Antitumor Potential in Sarcoma
    Article Snippet: .. Mice were injected intraperitoneally (i.p.) with 200 μg of specific monoclonal antibodies or inhibitors: anti-mouse CD8, InVivoPlus, clone YTS169.4 (BioXCell Cat. BP0117, RRID:AB_2894806) and the relative isotype control rat IgG2b, InVivoPlus, clone LTF-2 (BioXCell Cat. BP0090, RRID:AB_1107780); anti-mouse NK1.1, InVivoPlus, clone PK136 (BioXCell Cat. BP0036, RRID:AB_2894792) and the relative isotype control rat IgG2a, InVivoPlus, clone C1.18.4 (BioXCell Cat. BP0085, RRID:AB_1107771); anti-IFNInVivoPlus, clone XMG1.2 (BioXCell Cat. BP0055, RRID:AB_1107694) and the relative isotype control rat IgG1, InVivoPlus, clone HRPN (BioXCell Cat. BP0088, RRID:AB_1107775), the day before tumor cell transplantation. .. They were the injected i.p. with 100 μg once (anti-NK1.1), twice (anti-CD8) or three times (anti- IFNa week for the entire duration of the experiment; anti-mouse PD-1, InVivoPlus, clone RMP1- 14 (BioXCell, Cat. BP0146, RRID:AB_10949053) and the relative isotype control rat IgG2a, InVivoPlus, clone 2A3 (BioXCell, Cat. BP0089, RRID: AB_1107769), 200 μg twice a week, starting from 9 days after tumor cell injection.

    Injection:

    Article Title: IL-1R2 Deficiency Unleashes Neutrophil-Mediated Antitumor Potential in Sarcoma
    Article Snippet: .. Mice were injected intraperitoneally (i.p.) with 200 μg of specific monoclonal antibodies or inhibitors: anti-mouse CD8, InVivoPlus, clone YTS169.4 (BioXCell Cat. BP0117, RRID:AB_2894806) and the relative isotype control rat IgG2b, InVivoPlus, clone LTF-2 (BioXCell Cat. BP0090, RRID:AB_1107780); anti-mouse NK1.1, InVivoPlus, clone PK136 (BioXCell Cat. BP0036, RRID:AB_2894792) and the relative isotype control rat IgG2a, InVivoPlus, clone C1.18.4 (BioXCell Cat. BP0085, RRID:AB_1107771); anti-IFNInVivoPlus, clone XMG1.2 (BioXCell Cat. BP0055, RRID:AB_1107694) and the relative isotype control rat IgG1, InVivoPlus, clone HRPN (BioXCell Cat. BP0088, RRID:AB_1107775), the day before tumor cell transplantation. .. They were the injected i.p. with 100 μg once (anti-NK1.1), twice (anti-CD8) or three times (anti- IFNa week for the entire duration of the experiment; anti-mouse PD-1, InVivoPlus, clone RMP1- 14 (BioXCell, Cat. BP0146, RRID:AB_10949053) and the relative isotype control rat IgG2a, InVivoPlus, clone 2A3 (BioXCell, Cat. BP0089, RRID: AB_1107769), 200 μg twice a week, starting from 9 days after tumor cell injection.

    Article Title: Tissue-specific abundance of interferon-gamma drives regulatory T cells to restrain DC1-mediated priming of cytotoxic T cells against lung cancer
    Article Snippet: For DT-mediated cell depletion, DT (Sigma-Aldrich) was diluted in PBS (GIBCO) and administered intraperitoneally ( i.p. ) to deplete FoxP3 + Treg cells in FoxP3 DTR mice, FoxP3 DTR /WT and FoxP3 DTR / Ifngr1 −/− BMCs (1μg DT/mouse) or XCR1 + DC1 in XCR1 DTR mice, XCR1 DTR /WT and XCR1 DTR / H2-Ab1 −/− BMCs (0.5μg DT/mouse) at the indicated timepoints. .. For in vivo treatments, mice were injected with 100 μg αCD28 antibody i.p. (clone 37.51, Bio X Cell), 1.42x10 −11 mol IL-12 protein fused to murine serum albumin r.o. (IL-12-MSA, manufactured in-house) or 200 μg IFNγ antibody i.p. (clone XMG1.2, Bio X Cell) at the indicated timepoints. .. Cells were resuspended in FACS buffer (PBS (GIBCO) with 1% FBS (Atlanta Biologicals) and 2 mM EDTA (Invitrogen)) containing Fixable Viability Dye eFluor 780, eFluor 506 or eFluor 405 (eBioscience) to distinguish live and dead cells and αCD16/CD32 (clone 93, BioLegend) to prevent non-specific antibody binding, and incubated for 15 min at 4°C.

    Bioprocessing:

    Article Title: IL-1R2 Deficiency Unleashes Neutrophil-Mediated Antitumor Potential in Sarcoma
    Article Snippet: .. Mice were injected intraperitoneally (i.p.) with 200 μg of specific monoclonal antibodies or inhibitors: anti-mouse CD8, InVivoPlus, clone YTS169.4 (BioXCell Cat. BP0117, RRID:AB_2894806) and the relative isotype control rat IgG2b, InVivoPlus, clone LTF-2 (BioXCell Cat. BP0090, RRID:AB_1107780); anti-mouse NK1.1, InVivoPlus, clone PK136 (BioXCell Cat. BP0036, RRID:AB_2894792) and the relative isotype control rat IgG2a, InVivoPlus, clone C1.18.4 (BioXCell Cat. BP0085, RRID:AB_1107771); anti-IFNInVivoPlus, clone XMG1.2 (BioXCell Cat. BP0055, RRID:AB_1107694) and the relative isotype control rat IgG1, InVivoPlus, clone HRPN (BioXCell Cat. BP0088, RRID:AB_1107775), the day before tumor cell transplantation. .. They were the injected i.p. with 100 μg once (anti-NK1.1), twice (anti-CD8) or three times (anti- IFNa week for the entire duration of the experiment; anti-mouse PD-1, InVivoPlus, clone RMP1- 14 (BioXCell, Cat. BP0146, RRID:AB_10949053) and the relative isotype control rat IgG2a, InVivoPlus, clone 2A3 (BioXCell, Cat. BP0089, RRID: AB_1107769), 200 μg twice a week, starting from 9 days after tumor cell injection.

    Article Title: Memory CD8 T cells are vulnerable to chronic IFN-γ signals but not to CD4 T cell deficiency in MHCII-deficient mice
    Article Snippet: .. Antibody for in vivo experiments; Anti-mouse IFN- (clone XMG1.2) purchased from Bio X cell The following hybridomas were obtained from ATCC and monoclonal antibodies were produced in-house; Anti-mouse CD4 (clone GK1.5) Anti-mouse CD16/32 (FcR) (clone 2.4G2) Commercially available antibodies were validated by their respective manufacturers. ..

    Control:

    Article Title: IL-1R2 Deficiency Unleashes Neutrophil-Mediated Antitumor Potential in Sarcoma
    Article Snippet: .. Mice were injected intraperitoneally (i.p.) with 200 μg of specific monoclonal antibodies or inhibitors: anti-mouse CD8, InVivoPlus, clone YTS169.4 (BioXCell Cat. BP0117, RRID:AB_2894806) and the relative isotype control rat IgG2b, InVivoPlus, clone LTF-2 (BioXCell Cat. BP0090, RRID:AB_1107780); anti-mouse NK1.1, InVivoPlus, clone PK136 (BioXCell Cat. BP0036, RRID:AB_2894792) and the relative isotype control rat IgG2a, InVivoPlus, clone C1.18.4 (BioXCell Cat. BP0085, RRID:AB_1107771); anti-IFNInVivoPlus, clone XMG1.2 (BioXCell Cat. BP0055, RRID:AB_1107694) and the relative isotype control rat IgG1, InVivoPlus, clone HRPN (BioXCell Cat. BP0088, RRID:AB_1107775), the day before tumor cell transplantation. .. They were the injected i.p. with 100 μg once (anti-NK1.1), twice (anti-CD8) or three times (anti- IFNa week for the entire duration of the experiment; anti-mouse PD-1, InVivoPlus, clone RMP1- 14 (BioXCell, Cat. BP0146, RRID:AB_10949053) and the relative isotype control rat IgG2a, InVivoPlus, clone 2A3 (BioXCell, Cat. BP0089, RRID: AB_1107769), 200 μg twice a week, starting from 9 days after tumor cell injection.

    Transplantation Assay:

    Article Title: IL-1R2 Deficiency Unleashes Neutrophil-Mediated Antitumor Potential in Sarcoma
    Article Snippet: .. Mice were injected intraperitoneally (i.p.) with 200 μg of specific monoclonal antibodies or inhibitors: anti-mouse CD8, InVivoPlus, clone YTS169.4 (BioXCell Cat. BP0117, RRID:AB_2894806) and the relative isotype control rat IgG2b, InVivoPlus, clone LTF-2 (BioXCell Cat. BP0090, RRID:AB_1107780); anti-mouse NK1.1, InVivoPlus, clone PK136 (BioXCell Cat. BP0036, RRID:AB_2894792) and the relative isotype control rat IgG2a, InVivoPlus, clone C1.18.4 (BioXCell Cat. BP0085, RRID:AB_1107771); anti-IFNInVivoPlus, clone XMG1.2 (BioXCell Cat. BP0055, RRID:AB_1107694) and the relative isotype control rat IgG1, InVivoPlus, clone HRPN (BioXCell Cat. BP0088, RRID:AB_1107775), the day before tumor cell transplantation. .. They were the injected i.p. with 100 μg once (anti-NK1.1), twice (anti-CD8) or three times (anti- IFNa week for the entire duration of the experiment; anti-mouse PD-1, InVivoPlus, clone RMP1- 14 (BioXCell, Cat. BP0146, RRID:AB_10949053) and the relative isotype control rat IgG2a, InVivoPlus, clone 2A3 (BioXCell, Cat. BP0089, RRID: AB_1107769), 200 μg twice a week, starting from 9 days after tumor cell injection.

    other:

    Article Title: Tissue-specific abundance of interferon-gamma drives regulatory T cells to restrain DC1-mediated priming of cytotoxic T cells against lung cancer
    Article Snippet: Cell fixation was achieved using the Foxp3 Transcription Factor Fixation/Permeabilization buffer (eBioscience) when staining for transcription factors, or the Cytofix/Cytoperm Fixation/Permeabilization buffer (BD Biosciences) when staining for cytokines, following manufacturer’s instructions.

    In Vivo:

    Article Title: Tissue-specific abundance of interferon-gamma drives regulatory T cells to restrain DC1-mediated priming of cytotoxic T cells against lung cancer
    Article Snippet: For DT-mediated cell depletion, DT (Sigma-Aldrich) was diluted in PBS (GIBCO) and administered intraperitoneally ( i.p. ) to deplete FoxP3 + Treg cells in FoxP3 DTR mice, FoxP3 DTR /WT and FoxP3 DTR / Ifngr1 −/− BMCs (1μg DT/mouse) or XCR1 + DC1 in XCR1 DTR mice, XCR1 DTR /WT and XCR1 DTR / H2-Ab1 −/− BMCs (0.5μg DT/mouse) at the indicated timepoints. .. For in vivo treatments, mice were injected with 100 μg αCD28 antibody i.p. (clone 37.51, Bio X Cell), 1.42x10 −11 mol IL-12 protein fused to murine serum albumin r.o. (IL-12-MSA, manufactured in-house) or 200 μg IFNγ antibody i.p. (clone XMG1.2, Bio X Cell) at the indicated timepoints. .. Cells were resuspended in FACS buffer (PBS (GIBCO) with 1% FBS (Atlanta Biologicals) and 2 mM EDTA (Invitrogen)) containing Fixable Viability Dye eFluor 780, eFluor 506 or eFluor 405 (eBioscience) to distinguish live and dead cells and αCD16/CD32 (clone 93, BioLegend) to prevent non-specific antibody binding, and incubated for 15 min at 4°C.

    Article Title: Memory CD8 T cells are vulnerable to chronic IFN-γ signals but not to CD4 T cell deficiency in MHCII-deficient mice
    Article Snippet: .. Antibody for in vivo experiments; Anti-mouse IFN- (clone XMG1.2) purchased from Bio X cell The following hybridomas were obtained from ATCC and monoclonal antibodies were produced in-house; Anti-mouse CD4 (clone GK1.5) Anti-mouse CD16/32 (FcR) (clone 2.4G2) Commercially available antibodies were validated by their respective manufacturers. ..

    Article Title: IL-6 fuels durable memory for Th17 cell-mediated responses to tumors
    Article Snippet: .. In vivo blocking or neutralizing antibodies were purchased from BioXCell: (anti-IL-6, clone MP5-20F3; anti-IL-6R, clone 15A7; anti-IL-17A, clone 17F3; anti-IFN-γ, clone XMG1.2; anti-CD4, clone GK1.5; anti-CD8, clone 2.43; Isotype controls: IgG1 clone HRPN, IgG2b clone LTF-2, mouse IgG1, clone MOPC-21). .. M108 was injected subcutaneously in Matrigel (Corning) 47 days prior to ACT.

    Neutralization:

    Article Title: Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity.
    Article Snippet: Depletions of immune cells were done using antibodies against CD8 (clone 2.43, BioXCell 400 ug i.p. twice weekly), NK1.1 (clone PK136, BioXCell, 400 ug i.p. twice weekly), Ly6g (clone 1A8, BioXCell, 400 ug i.p. twice weekly) or CSF1R (clone AFS98, BioXCell, 300 ug i.p. every other day) as previously described. .. Cytokine neutralization was done using i.p. treatments with 200 ug of antibodies against IFN- (clone XMG1.2, BioXCell), IL-1b (clone B122, BioXCell) and IL-18 (clone YIGIF74-1G7, BioXCell) every other day. .. Validation All antibodies other than TA99 were validated by the vendors: Biolegend, BD Biosciences, ThermoFisher, Abcam and BioXCell.

    Produced:

    Article Title: Memory CD8 T cells are vulnerable to chronic IFN-γ signals but not to CD4 T cell deficiency in MHCII-deficient mice
    Article Snippet: .. Antibody for in vivo experiments; Anti-mouse IFN- (clone XMG1.2) purchased from Bio X cell The following hybridomas were obtained from ATCC and monoclonal antibodies were produced in-house; Anti-mouse CD4 (clone GK1.5) Anti-mouse CD16/32 (FcR) (clone 2.4G2) Commercially available antibodies were validated by their respective manufacturers. ..

    Blocking Assay:

    Article Title: IL-6 fuels durable memory for Th17 cell-mediated responses to tumors
    Article Snippet: .. In vivo blocking or neutralizing antibodies were purchased from BioXCell: (anti-IL-6, clone MP5-20F3; anti-IL-6R, clone 15A7; anti-IL-17A, clone 17F3; anti-IFN-γ, clone XMG1.2; anti-CD4, clone GK1.5; anti-CD8, clone 2.43; Isotype controls: IgG1 clone HRPN, IgG2b clone LTF-2, mouse IgG1, clone MOPC-21). .. M108 was injected subcutaneously in Matrigel (Corning) 47 days prior to ACT.



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